Table 1.

Binding affinity constants (K_AS) of biotin-labelled cap analogues for murine eIF4E.

cap analogue	KAS (µM−1)	ΔGc (kcal mol−1)
m7GpppG	9.4 ± 0.4b	−9.35 ± 0.03
m72′-N-BiotGpppG (1)	3.8 ± 0.1b	−8.82 ± 0.02
m72′-N-BiotGpNHppG (2)	3.3 ± 0.1a	−8.74 ± 0.02
m72′-N-BiotGppSpG (3)	5.7 ± 0.2a	−9.06 ± 0.02
m72′-N-BiotGppNHpG (4)	3.0 ± 0.2a	−8.68 ± 0.02
m72′-N-BiotGppCH2pG (5)	3.6 ± 0.2a	−8.79 ± 0.02

^aConditions for FQT experiments: 50 mM HEPES/KOH, 100 mM KCl, 0.5 mM EDTA, pH 7.2.

^bData from Jemielity et al. [14].