Table 2.
In vitro biochemical properties of cap analogues assessed in this study.
| cap analogue | capping efficiency (%) | translation efficiency (RRL) | hDcp2 decapping susceptibility |
|---|---|---|---|
| m7GpppG | 76 | 1 | fast degradation |
| m27,2′-OGpppG (ARCA) | 69 | 1.56 ± 0.48 | fast degradation |
| ApppG | 43 | 0.03 ± 0.01 | resistant |
| m72′-N-BiotGpppG (1) | 61 | 0.68 ± 0.16 | slow degradation |
| m72′-N-BiotGpNHppG (2) | 64 | 0.37 ± 0.12 | slow degradation |
| m72′-N-BiotGppSpG (3) | 61 | 0.93 ± 0.14 | partially resistant |
| m72′-N-BiotGppNHpG (4) | 39 | 0.11 ± 0.04 | resistant |
| m72′-N-BiotGppCH2pG (5) | 47 | 0.28 ± 0.08 | resistant |