Fig. 2.

Downregulation of MPC1/2 attenuates IFNγ-induced apoptosis. (A) Co-Overexpression of murine and human MPC1 and MPC2 in MC38 and CaCO-2 cancer cells. The overexpression was detected by western-blot. (B) After treated vector and MPC1/2 overexpressing MC38 and CaCO-2 cells with PBS or IFNγ (50 ng/ml) for 48 h, the cell lysates were extracted and the levels of full lenghth Caspase 7 (CAS7) and Caspase 3 (CAS3) and cleaved Caspase 7 (C-CAS7) and cleaved Caspase 3 (C-CAS3) were detected by western-blot and β-actin was used as internal control. (C) Vector and MPC1/2 overexpressing MC38 and CaCO-2 cells were treated with PBS or IFNγ (50 ng/ml) for 48 h, then these cells were trypsinized and stained with Annexin-V and 7-AAD. The overall apoptosis was detected with flow cytometry. Data shown are representative of three independent experiments and error bars represent mean ± s.e.m.; *p < 0.05, (Student's t-test).