Subject area:	Biochemistry, Genetics and Molecular Biology
More specific subject area:	Biophysics of lipids
Method name:	Quantitative imaging of cell micropolarity
Name and reference of original method:	G. Maulucci, F. Di Giacinto, C. De Angelis, O. Cohen, B. Daniel, C. Ferreri, M. De Spirito, S. Sasson, Real time quantitative analysis of lipid storage and lipolysis pathways by confocal spectral imaging of intracellular micropolarity, Biochim. Biophys. Acta - Mol. Cell Biol. Lipids. 1863 (2018) 783–793. doi:10.1016/J.BBALIP.2018.04.004.
Resource availability:	The PhasorM program and the supplementary data and materials are available in Mendeley Data at the following link: https://data.mendeley.com/datasets/z3g572hvjs/draft?a=01d35be1-2cf2-4223-ba09-e4f15375de5b
Application file for the phasor analysis (Windows)	PhasorM Windows.zip. The archive contains: PhasorM_installer.exe Application file for the phasor analysis (Mac OS): PhasorM MacOS.zip The archive contains: MyAppIInstaller_mcr
Sample data and mask	supplementary materials.zip The archive contains: A folder “data” which contains: 1. Reference lipids TO.tif: 32 channel spectral image of nile red labelled Triolein 2. Spectral image cells.tiff: 32 channel spectral image of Nile red labelled cells A folder “masks” which contains 3. The files of the masks which can be directly used for the analysis (NP_Mask_95_noOverlap.mat,P_Mask_95_noOverlap.mat and HP_Mask_95_noOverlap.mat) if these requirements are met: 32 channels spectral detector in the range 580-660 nm and an excitation laser of 488 nm wavelength.