FIGURE 3.

BBB transporters, tight and adherens junction mRNA levels and protein expression are attenuated upon PX exposure. (A) Monolayers of BLECs were treated with PX for 24 h and the mRNA expression levels of the junctional genes Occludin, Claudin-5, ZO-1, Ve-Cadherin, and transporter genes Glut-1 and MDR-1 were examined (n = 3 with 8–9 technical repeats). (B) ECs monolayers were treated with PX for 24 h and protein expression levels and patterns of Claudin-5, ZO-1, Ve-Cadherin and P-gp were examined using immunocytochemistry, one representative picture is displayed. Bar scales for two top panels equal 50 and 20 μm for the third row. Magnification level for the bottom panels is x200. Data presented as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 vs. control (Kruskal-Wallis one way ANOVA with Dunnett’s multiple comparisons test).