FIGURE 4.

Cell area enlargement as an endothelium compensatory mechanism to maintain barrier properties by PX. (A) Dose-dependent cell area expansion in monolayers of BLECs treated for 24 h with PX calculated with imageJ from TJ-immunostained BLECs from at least 9 micrographs (n = 146–170 cells per treatment from three independent experiments). (B) A dying BLEC (marked with an arrow) covered by neighboring cells with functional adherens junctions at 300 μM PX (immunostaining of VE-cadherin is shown). Data presented as mean ± SEM. ^∗∗∗p < 0.001 vs. control (Ordinary one way ANOVA with Dunnett’s multiple comparisons test). Bar scale equal 20 μm.