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. 2018 Oct 16;9:2482. doi: 10.3389/fmicb.2018.02482

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Copyright © 2018 Brown, Wasala, Howe, Peetz, Zasada and Denver.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of Cardinium cPpe in whole body and ovaries of P. penetrans by FISH and confocal microscopy. (A–C) Split panels showing nematode nuclei (blue DAPI stain) alone (top), cPpe (yellow FISH probe) alone (middle), and combined images (bottom). (A) Whole female with o = ovaries, and yellow autofluorescence of lip and spermathecal regions. (B) Female with combined brightfield DIC image (bottom), v = vulva. (C) High magnification of (A) showing ovaries and developing oocytes (arrows), v = vulva. Scale bars = 20 μm.