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. 2018 Oct 16;9:1520. doi: 10.3389/fpls.2018.01520

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Copyright © 2018 Zhang, Yue, Zheng, Gautam, He and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ChIP analysis. It revealed that the modification of H3K9ac and H4K5ac in the promoter region of ZmHO-1 and ZmGSL-1 genes was significantly altered under the heat stress. (A) The level of H3K9ac and H4K5ac in the upstream region of ZmHO-1 gene was significantly decreased under heat treatment. (B) The level of H3K9ac in the upstream region of ZmGSL-1 gene was significantly decreased and H4K5ac was found to be slightly present on the set B after 3 days of treatment, whereas it increased significantly on the set C after 6 days of treatment. The acetylation level of the control group was set to 1.0 at each time point. The relative abundance of histone modification was repeated three times. Asterisk (^∗) indicated that the relative increase of H3K9ac or H4K5ac under heat treatment group is significantly different from that of the control group (t-test, p < 0.01).