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. 2018 Oct 16;9:2387. doi: 10.3389/fimmu.2018.02387

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Copyright © 2018 Fu, Thorpe, Akula, Chahal and Hellman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromogenic substrate assay. (A–K) A panel of different chromogenic substrates was used to determine the primary specificity of hNE, HPR-3, xPR-3, hCG, human granzyme B, and human thrombin. The panel included different chymase, elastase, tryptase and aspase substrates. The amino acid sequences of the substrates are listed on the left side of the figure. Human thrombin, hCG, and human granzyme B were included as reference enzymes for tryptase, chymase, and asp-ase activities, respectively.