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. 2018 Oct 16;12:84. doi: 10.3389/fnana.2018.00084

FIGURE 2.

FIGURE 2

Control experiments for rabies-mediated transsynaptic tracing. (A) Injection of RV without prior AAV injection resulted in no dsRed-labeled neurons, indicating the dependence of the RV infection on AAV-induced expression of TVA. Scale bar, 500 μm. (B) Injecting AAV-DIO-EGFP-TVA, AAV-DIO-RG, and RV into the wild-type mice led to a low level of nonspecific labeled RV neurons that expressed dsRed at the injection site. Scale bar, 500 μm. (C1) Injection of AAV-DIO-EGFP-TVA and RV without prior AAV-DIO-RG injection in the D1R-Cre mice resulted in no DsRed-labeled input neurons. Scale bar, 500 μm. (C2) Enlarged view of the boxed region in panel (C1); all dsRed-positive neurons coexpressed eGFP. Scale bar, 50 μm.