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. 2018 Oct 29;9:2501. doi: 10.3389/fimmu.2018.02501

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Copyright © 2018 Bottero, Zurita, Gaillard, Carriquiriborde, Martin Aispuro, Elizagaray, Bartel, Castuma and Hozbor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of OMVs by electron microscopy and electrophoresis. (A) Transmission-electron-microscopy image of the negatively stained preparations obtained in the virulent phase from B. parapertussis strains AR729 (OMVs(Bpp-LPS-O⁺)) and ATCC 15237 (OMVs(Bpp-LPS-O⁻)). Scale bar: 200 nm. (B) LPS analysis of OMVs(Bpp-LPS-O⁺) and OMVs(Bpp-LPS-O⁻) by 15% (w/v) SDS-PAGE. The bands were visualized by the BioRad silver-staining technique. In the figure, the sources of the samples are indicated either above (A) or below (B) the representative illustrations. (C) Immunoblotting of purified B. parapertussis LPS separated by 15% (w/v) SDS-PAGE probed with the specific O antigen polyclonal antiserum obtained from mice. The arrows indicate the locations of the O antigen (O-Ag) and the Band B.