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. 2018 Oct 23;10:36. doi: 10.3389/fnsyn.2018.00036

Figure 2.

Figure 2

mGlu1-LTD at NAc glutamatergic synapses onto MSN recorded from rats that underwent >35 days of cocaine withdrawal (>WD35) requires the dynamin-dependent endocytosis of CP-AMPARs. (A) DHPG-induced attenuation of locally-evoked EPSC−70 mV in NAc MSNs was no longer observed when the dynamin inhibitory peptide (DIP, 2 mM) was included in the recording electrode (n = 6 cells/5 rats). Data from control internal solution recordings (from Figure 1B) were included for comparison. Inset bar graph summarizes the magnitude of EPSC suppression obtained during the last 5 min of DHPG washout (**p < 0.001, unpaired t-test). (B) While the inclusion of DIP in the recording electrode blocked the expression of DHPG-induced LTD, it did not disrupt the inhibitory effect of the CP-AMPAR antagonist 1-naphthyl acetyl spermine (NASPM; 100 μM) on MSN EPSC−70 mV amplitude (n = 7 cells/7 rats). Inset bar graph summarizes the extent of EPSC suppression recorded during the last 5 min of DHPG bath application alone and with the addition of NASPM (**p < 0.001 vs. baseline, paired t-test). Calibration bars for example traces: 100 pA, 25 ms.