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. 2018 Oct 18;9:1187. doi: 10.3389/fphar.2018.01187

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Copyright © 2018 Chen, Zhu, Sun and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of astilbin on HG-induced the expression of TGF-β1, CTGF, MMP-2, and MMP-9. HBZY-1 cells were treated with 10 and 20 μg/ml of astilbin under HG condition for 24 h. The mRNA levels of TGF-β1 (A) and CTGF (B) in HBZY-1 cells were detected by qRT-PCR. The secretion levels of TGF-β1 (C) and CTGF (D) in cell supernatant were detected by ELISA. (E) The expression levels of MMP-2 and MMP-9 were determined by western blot. ^#P < 0.05 vs. Con (control group). ^∗P < 0.05 vs. HG-stimulated group. Statistical significance was determined by one-way ANOVA.