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. 2018 Nov 6;9:1620. doi: 10.3389/fpls.2018.01620

FIGURE 4.

FIGURE 4

bri1-301 is sensitive to BL at lower temperature but insensitive to BL at higher temperature. (A) Root growth inhibition assay of Col-0 and bri1-301 at 18°C, 22°C, and 28°C. Seven-day-old seedlings grown on 1/2 MS media supplemented with or without 1 μM 24-epiBL were photographed. Scale bar represents 1 cm. (B) Measurements of primary root length of the seedlings as shown in (A). The asterisks indicate statistical significance evaluated by Student’s t-test (∗∗P < 0.01), ns represents not significant. Three independent biological replicates were carried out. Similar results were obtained. One of the representative results is shown. (C,D) Transcriptional responses of CPD (C) and SAUR-AC1 (D) to 24-epiBL treatment at three different temperatures. ACTIN2 was used as an internal control. Three independent biological replicates were carried out. Similar results were obtained. One of the representative results is shown. (E) BES1 phosphorylation analysis in response to exogenous 24-epiBL at different temperatures. The upper and lower bands represent phosphorylated and dephosphorylated BES1, respectively. The middle bands marked by an asterisk indicate non-specific signals generated by anti-BES1 serum. Tubulin was used as the loading control.