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. 2018 Nov 6;9:1620. doi: 10.3389/fpls.2018.01620

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Copyright © 2018 Lv, Li, Chen, Wang, Sun, Yin, He and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stability of bri1-301 protein is negatively regulated by elevated temperature. (A) Western blot analyses of total protein extracts from aerial parts of 3-week-old pBRI1::BRI1-FLAG bri1-701 and pBRI1::bri1-301-FLAG bri1-701 transgenic plants. Tubulin was used as the loading control. (B) Relative BRI1 or bri1-301 protein levels were calculated as the average ratios (±SD) of Bri1/Tubulin signals in (A) from three independent experiments. (C) BRI1 and bri1-301 protein accumulation under short-term warm treatment. Three-week-old 18°C-grown pBRI1::BRI1-FLAG bri1-701 and pBRI1::bri1-301-FLAG bri1-701 transgenic plants were transferred into a 28°C growth chamber and the aerial parts were harvested at the indicated time points. Tubulin was used as the loading control. Three independent biological replicates were carried out. Similar results were obtained. One of the representative results is shown. (D,E) Abundance of BRI1 (D) and bri1-301 (E) in response to a time-course CHX treatment at three temperatures. Seven-day-old pBRI1::BRI1-GFP bri1-701 and pBRI1::bri1-301-GFP bri1-701 transgenic seedlings were used for the CHX treatment, total protein extracts were used for Western blot analyses. Tubulin was used as the loading control. Relative BRI1 or bri1-301 protein levels were calculated as ratios of BRI1/Tubulin.