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. 2018 Aug 15;29(17):2037–2044. doi: 10.1091/mbc.E18-02-0096

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© 2018 Fu et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Inhibitory effect of extraordinary tight-binding NESs. (A) EYFP₂-SV40^NLS-PKI^NES is cotransfected with RFP, RFP-MVM NS2^NES, RFP-Super PKI^NES, RFP-PKI^NES, or RFP- X11L2^NES and compared with cells expressing EYFP₂-SV40^NLS-PKI^NES that are treated with small molecule inhibitor LMB. (B) EYFP₂-SV40^NLS-X11L2^NES is cotransfected with RFP, RFP-MVM NS2^NES, RFP-Super PKI^NES, RFP-PKI^NES, or RFP-X11L2^NES and compared with cells expressing EYFP₂-SV40^NLS-PKI^NES that are treated with small molecule inhibitor LMB. The numbers of examined cells from at least three independent experiments are indicated in parentheses in A and B. Error bars represent SD. The p values were calculated in comparison to control (reporter only) using Mann–Whitney tests. Note that observed R_C/N values are presented without normalization to compare with R_C/N on LMB treatment (+LMB). (C) Representative images of cells transfected with EYFP₂-SV40^NLS-PKI^NES (reporter) and RFP-tagged NESs (competitive peptides). YFP (pseudocolored in yellow), Hoechst (pseudocolored in blue), and RFP (pseudocolored in red) images were captured using spinning disk confocal microscope (40×). The expression levels of reporter proteins and competitive peptides and are summarized in Supplemental Figure S1C.