FIGURE 7:

UBC-13 functions in EGL-20/Wnt–producing cells to regulate MIG-14 trafficking and Wnt signaling. (A–A″′) DIC and fluorescence images of EGL-20–producing cells (dashed circles) coexpressing GFP and CHERRY driven by ubc-13 and egl-20 promoters, respectively. (B–E) Confocal fluorescence images of MIG-14::GFP driven by the egl-20 promoter in wild type (WT; B), ubc-13(tm3546) (C), vps-29(tm1320) (D), and vps-29;ubc-13 (E). Arrowheads indicate the plasma membrane labeled by MIG-14::GFP. (F, G) Quantification of MIG-14::GFP on plasma membranes (F) and in the cytosol (G) of EGL-20–producing cells in the indicated strains. At least eight animals were quantified. (H) Fluorescence images of GFP controlled by the mec-4 promotor in wild type and egl-20(n585). The two Q neuroblast descendants AVM and PVM are labeled by GFP. Arrowheads point to PVM cells, and asterisks indicate the vulva region. (I) The migration of QL.ds was quantified in the indicated strains. At least 100 worms were quantified. In F, G, and I, data are shown as mean ± SD. One-way ANOVA with Tukey’s post hoc test was performed to compare data sets that are linked by lines. ***, P < 0.0001. Scale bars indicate 5 μm in A–E and 50 μm in H.