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. 2018 Aug 15;29(17):2084–2097. doi: 10.1091/mbc.E18-03-0192

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© 2018 Qadota et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — Mapping of interaction sites of PPTR-1 and PPTR-2 for UNC-89, and of UNC-89 for PPTR-1 and PPTR-2. (A) When tested by yeast two-hybrid assays with segments that cover all of UNC-89B, PPTR-1 and PPTR-2 only interact with UNC-89 1/3 IK-Ig53-Fn2. Pink rectangles, Ig domains; green rectangles, Fn3 domains; other domains are as indicated. (B) Domain mapping using yeast two-hybrid assays indicates that the minimal region of UNC-89 that interacts with PPTR-1 is 1/3 IK-Ig53-Fn2, but that the minimal region of UNC-89 that interacts with PPTR-2 is Ig53-Fn2.