Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 15;29(17):2055–2068. doi: 10.1091/mbc.E18-01-0007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Shi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 8: — Caly-WT but not Caly-ATEA increases the motility of PI4KIIα-labeled organelles. (A) Left panels, colocalization of GFP-PI4KIIα (green) and mCh-Caly-WT (red). Arrows point to overlapping puncta, and enlarged box show some examples of nonoverlapping puncta. Right panels, representative kymographs of PI4KIIα-labeled organelles in axons transfected with mCherry (mCh), mCh-Caly-WT (Caly-WT), and mCh-Caly-ATEA (Caly-ATEA). (B) Average speeds and run lengths of both anterograde and retrograde motile events are higher in Caly-WT axons, whereas values detected in Caly-ATEA axons do not differ from control. (C) Box-and-whisker plots show that retrogradely moving PI4KIIα-labeled organelles are more common in Caly-WT axons relative to static and bidirectional organelles. (D) No difference in retrograde and anterograde motile events over >10 μm were detected. Data shown in bar graphs (mean ± SEM) and box-and-whisker plots (mean ± 95% CI) reflect results obtained in three independent experiments, with at least five axons per group in each experiment; *p < 0.05, **p < 0.01, ***p < 0.001; A.S., axon segment; bar = 20 μm.