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. 2018 Aug 8;29(16):1941–1947. doi: 10.1091/mbc.E18-01-0061

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© 2018 Yamashiro, Tanaka, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 1: — (A) Live image of DyLight550-actin (DL-actin) speckles in a keratocyte with epifluorescence microscopy. (B) Positions of appearance of DL-actin speckles in a 34-s time window (left) and disappearance of these speckles (right) are indicated by pink dots over an image of the keratocyte. Bars, 10 μm. (C) Lifetime distribution of DL-actin speckles in lamellipodia of keratocytes.