Table 2. Effects of methanol, acetone and ethyl acetate extracts of Pseudevernia furfuracea and Platismatia glauca on the stage of cell death in HCT-116 and SW-480 cells.
| t/h | γ/(µg/mL) | Viable cells/% | Early apoptosis/% | Late apoptosis/% | Necrosis/% | |||
|---|---|---|---|---|---|---|---|---|
| Control | 24 | 0 | 97.4±3.6 | 2.6±0.4 | 0 | 0 | ||
| 72 | 0 | 95.4±1.4 | 4.56±0.03 | 0 | 0 | |||
| HCT-116 | P. furfuracea | methanol | 24 | 10 | 79.1±1.1 | 10.5±0.1 | 10.3±0.1 | 0 |
| 100 | 65.2±2.1 | 16.5±0.5 | 18.3±1.0 | 0 | ||||
| 72 | 10 | 73.6±2.7 | 16.1±0.7 | 10.1±0.9 | 0.2±0.0 | |||
| 100 | 31.7±2.0 | 39.5±0.5 | 28.84±0.05 | 0 | ||||
| acetone | 24 | 10 | 66.5±1.0 | 13.6±1.0 | 19.9±0.2 | 0 | ||
| 100 | 28.8±1.6 | 21.6±0.3 | 49.6±0.1 | 0 | ||||
| 72 | 10 | 61.4±0.4 | 18.0±0.4 | 20.5±0.5 | 0.1±0.0 | |||
| 100 | 18.4±0.1 | 18.1±0.9 | 58.47±0.06 | 5.06±0.03 | ||||
| ethyl acetate |
24 | 10 | 62.8±0.4 | 17.8±0.8 | 18.6±0.7 | 1.17±0.02 | ||
| 100 | 28.3±0.5 | 21.12±0.01 | 50.07±0.03 | 0.48±0.05 | ||||
| 72 | 10 | 38.5±1.1 | 41.4±0.1 | 20.1±0.0 | 0.28±0.01 | |||
| 100 | 8.0±0.1 | 10.9±0.4 | 75.4±0.2 | 5.62±0.01 | ||||
| P. glauca | methanol | 24 | 10 | 73.7±0.1 | 15.3±0.0 | 11.0±0.2 | 0 | |
| 100 | 57.1±0.1 | 11.9±0.3 | 38.1±0.4 | 0 | ||||
| 72 | 10 | 60.1±0.4 | 31.5±0.7 | 18.3±1.0 | 0 | |||
| 100 | 19.7±0.7 | 37.9±0.1 | 42.4±0.8 | 0 | ||||
| acetone | 24 | 10 | 68.0±0.4 | 12.5±0.1 | 15.2±0.5 | 4.2±0.1 | ||
| 100 | 40.0±1.4 | 26.8±0.5 | 26.17±0.08 | 7.4±1.1 | ||||
| 72 | 10 | 46.7±2.0 | 22.4±0.1 | 22.0±0.2 | 8.8±0.1 | |||
| 100 | 20.1±0.1 | 37.0±0.3 | 32.7±0.5 | 10.2±0.5 | ||||
| ethyl acetate |
24 | 10 | 76.5±1.8 | 13.2±0.5 | 9.02±0.08 | 1.21±0.01 | ||
| 100 | 74.15±0.01 | 30.73±0.01 | 13.1±0.3 | 12.0±0.8 | ||||
| 72 | 10 | 45.5±0.7 | 21.5±0.7 | 22.2±0.8 | 10.78±0.03 | |||
| 100 | 20.62±0.01 | 28.2±1.0 | 29.5±0.7 | 21.6±0.3 | ||||
| Control | 24 | 0 | 96.8±1.8 | 3.2±0.0 | 0 | 0 | ||
| 72 | 0 | 94.4±1.1 | 5.6±0.1 | 0 | 0 | |||
| SW-480 | P. furfuracea | methanol | 24 | 10 | 75.9±1.5 | 14.1±1.0 | 9.99±0.01 | 0 |
| 100 | 26.6±0.6 | 17.5±0.4 | 55.8±0.5 | 0 | ||||
| 72 | 10 | 82.1±0.8 | 15.3±1.1 | 8.66±0.01 | 0 | |||
| 100 | 18.0±0.1 | 13.9±0.1 | 60.6±0.9 | 7.49±0.04 | ||||
| acetone | 24 | 10 | 83.1±1.0 | 8.2±1.3 | 8.77±0.09 | 0 | ||
| 100 | 40.4±0.1 | 24.8±0.5 | 34.78±0.09 | 0 | ||||
| 72 | 10 | 70.2±1.2 | 18.8±0.8 | 10.9±0.0 | 0 | |||
| 100 | 12.0±0.0 | 14.2±0.8 | 69.6±1.7 | 4.1±0.4 | ||||
| ethyl acetate |
24 | 10 | 86.4±1.1 | 8.3±0.8 | 5.28±0.02 | 0 | ||
| 100 | 81.0±0.1 | 10.5±0.8 | 8.5±0.3 | 0 | ||||
| 72 | 10 | 80.73±0.09 | 11.0±0.8 | 8.24±0.01 | 0 | |||
| 100 | 24.82±0.08 | 5.6±0.9 | 66.1±0.5 | 3.49±0.04 | ||||
| P. glauca | methanol | 24 | 10 | 31.3±0.4 | 22.2±1.0 | 33.15±0.09 | 13.3±0.1 | |
| 100 | 22.6±0.1 | 31.58±0.4 | 27.4±0.6 | 18.4±1.0 | ||||
| 72 | 10 | 87.5±0.7 | 9.67±0.05 | 5.59±0.02 | 2.81±0.02 | |||
| 100 | 64.2±0.8 | 16.8±0.7 | 8.7±0.4 | 10.3±0.8 | ||||
| acetone | 24 | 10 | 44.1±0.5 | 25.4±1.0 | 15.15±0.04 | 15.3±0.1 | ||
| 100 | 18.8±0.1 | 32.3±0.4 | 28.4±0.6 | 20.4±1.0 | ||||
| 72 | 10 | 77.8±0.6 | 12.9±0.9 | 6.70±0.01 | 2.6±0.0 | |||
| 100 | 49.0±0.1 | 24.4±0.8 | 20.58±0.05 | 6.0±0.4 | ||||
| ethyl acetate |
24 | 10 | 61.5±0.9 | 8.8±0.01 | 24.60±0.04 | 5.1±0.1 | ||
| 100 | 50.5±0.7 | 18.4±1.0 | 15.6±0.7 | 15.52±0.09 | ||||
| 72 | 10 | 78.8±1.0 | 9.03±0.07 | 7.10±0.05 | 5.1±0.6 | |||
| 100 | 49.0±0.7 | 22.1±0.3 | 10.5±1.0 | 18.4±0.1 | ||||
Results are expressed as the mean value±S.E. of three independent experiments