Table 5. Specific inhibitions of the radicals ABTS+ and DPPH, and the enzyme activity of peroxidase and amylase in natural extracts and synthetic mixtures of microalgae.
Parameter | Spirulina sp. | Synthetic Spirulina sp. | Nannochloropsis sp. | Synthetic Nannochloropsis sp. | ||||
---|---|---|---|---|---|---|---|---|
Inhibition %/(min·μg) |
CV/% | Inhibition %/(min·μg) |
CV/% | Inhibition %/(min·μg) |
CV/% | Inhibition %/(min·μg) |
CV/% | |
γ(phenolics)/(μg/mL) | ABTS inhibition | |||||||
3.0 | 1.15b | 7.8 | 0.40d | 9.3 | 1.73a | 9.2 | 0.72c | 4.4 |
5.0 | 0.96b | 2.7 | 0.40d | 7.3 | 1.33a | 10.1 | 0.68c | 2.8 |
10.0 | 0.93a | 7.7 | 0.35c | 5.7 | 0.95a | 12.3 | 0.62b | 1.0 |
Linear regression | y=5.1269x+4.5282 | – | y=1.8983x+1.941 | – | y=3.6239x+21.367 | – | y=3.4845x+2.7872 | – |
IC50/(μg/mL) | 9.0 | – | 25 | – | 8.0 | – | 14 | – |
t/min | DPPH inhibition | |||||||
30 | 0.003c | 20.7 | 0.014a | 2.2 | 0.026b | 12.0 | 0.028a | 7.5 |
60 | 0.006b | 5.3 | 0.015a | 1.9 | 0.015a | 3.5 | 0.015a | 5.5 |
90 | 0.007b | 3.5 | 0.014ab | 1.1 | 0.011a | 10.1 | 0.010ab | 7.4 |
120 | 0.007b | 1.6 | 0.013ab | 1.0 | 0.010a | 11.7 | 0.008ab | 8.5 |
150 | 0.007b | 0.8 | 0.012ab | 0.8 | 0.009a | 12.9 | 0.007b | 12.4 |
180 | 0.007ab | 1.3 | 0.013ab | 0.0 | 0.008a | 15.8 | 0.006b | 14.0 |
t/min | Peroxidase activity inhibition | |||||||
10 | 0.49b | 1.5 | 0.49b | 2.8 | 0.59a | 5.1 | 0.45b | 9.7 |
20 | 0.36a | 3.7 | 0.39a | 2.4 | 0.39a | 3.0 | 0.37a | 1.7 |
30 | 0.27a | 0.6 | 0.28a | 0.6 | 0.28a | 2.0 | 0.28a | 0.3 |
Amylase activity inhibition | ||||||||
0.075a | 0.7 | 0.012b | 10.8 | 0.010b | 4.9 | – | – |
For each method, average values followed by the same letters in the same row do not differ statistically according to Tukey’s test (p<0.05), CV=coefficient of variation