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. 2018 Nov 6;9:1239. doi: 10.3389/fphar.2018.01239

Figure 7.

Figure 7

MF treatment improved bone quality through regulation of AGEs/RAGE/Nox4/NF-κB signaling in the tibias and femurs of diabetic rats. (A,B) Show the representative micro-images of IHC staining (sections were counterstained with hematoxylin; original magnification, × 100, Scale bar: 200 μm; × 200, Scale bar: 100 μm; n = 10) and their analyses of AGEs expressions in the femurs of different groups of rats. (C–G) Show the representative images of western blot and their analyses of protein expressions of AGEs, RAGE, Nox4, and NF-κB in the tibias of different groups of rats (n = 10). The dark brown particles denote positive staining. (H,I) Show the serum levels of IL-6 and TNF-α in the different groups of rats (n = 12). IOD denotes integrated optical density of the interested areas. Data are presented as mean ± SD. #p < 0.05 or ##p < 0.01 compared with NC group, *p < 0.05 or **p < 0.01 compared with DM group, Δp < 0.05 or ΔΔp < 0.01 compared with MFL group. The expression of AGEs, RAGE, Nox4, and NF-κB were detected by stripping and redeveloping the same membrane with different primary antibodies. The amounts of protein in each lane was verified by redeveloping the membrane with β-actin.