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. 2018 Oct 1;29(20):2397–2409. doi: 10.1091/mbc.E18-06-0380

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© 2018 Tiengwe, Koeller, and Bangs. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 1: — Schematic representation of E6 reporters. Diagrams depict RNAi^R GPI-anchored (HA:E6) and GPI-minus (HA:E6ΔG) HA-tagged ESAG6 reporters. N-terminal gray boxes (ss) denote the EP1 Procyclin signal sequence (not to scale); arrowhead indicates position of HA epitope tag (3xHA); filled lollipops indicate N-linked glycan sites; C-terminal gray box (gpi) signifies the GPI attachment peptide (not to scale); underbar indicates the E6 RNAi^R insert with flanking XhoI (X) and BamHI sites (B).