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. 2018 Oct 1;29(20):2397–2409. doi: 10.1091/mbc.E18-06-0380

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© 2018 Tiengwe, Koeller, and Bangs. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 3: — Location of E6 reporters under TfR silencing. IFA was performed post–TfR RNAi induction with the HA:E6 (A)- or HA:E6ΔG (B)-expressing cell lines. Staining of fixed permeabilized cells was performed with mAb anti-HA (left, red), and with rabbit anti-BiP or rabbit anti-VSG (middle, green). Three-channel merged images with DAPI staining (blue) are presented (right). In each case, representative deconvolved summed stacked projections are presented. Cell outlines were traced from matched DIC images.