FIGURE 4:

Time course of TNFα-induced JNK activation. (A–E) WT and WDR62-KO cells were treated with TNFα (50 ng/ml) for the indicated time points. Following stimulation, cells were harvested and subjected to Western blot analysis and densitometric analysis with the indicated antibodies. p-JNK level is expressed relative to total JNK (B); p-Jun, c-Jun, and IκBα levels are expressed relative to α-tubulin (C–E). The ratio of p-JNK/JNK, p-Jun/α-tubulin, c-Jun/α-tubulin, and IκBα/α-tubulin obtained with untreated WT cells was determined as 1, while the ratio obtained with all other samples was determined relatively. Results are expressed as the mean ± SEM of three independent experiments. *p ≤ 0.05 comparing between WT and WDR62-KO counterparts. (F) WT and WDR62-KO cells were treated with TNFα (50 ng/ml) for 30 min. Then, mRNA was extracted and the indicated genes were analyzed by qRT-PCR. The expression level of GAPDH gene was used to normalization. Results are expressed as the mean ratio ± SEM of three independent experiments. *p ≤ 0.05 comparing treated to untreated cells of the same genotype; #p ≤ 0.05 comparing between WT and WDR62-KO counterparts.