CSU4 acts upstream of PIF4. (A–D) Hypocotyl phenotypes and length of 5-d-old Col, csu4-1, pif4-2, and csu4-1 pif4-2 mutant seedlings grown in W (14.74 μmol⋅m−2⋅s−1) (A and B) or R (129 μmol⋅m−2⋅s−1) (C and D) conditions. (E–H) Hypocotyl phenotypes and length of 5-d-old Col, csu4-1, cca1-22, and csu4-1 cca1-22 mutant seedlings grown in LD (E and F) or SD (G and H) conditions. The unit of hypocotyl length is millimeters. Error bars represent SE (n ≥ 20). Letters above the bars indicate significant differences (P < 0.05) as determined by one-way ANOVA with Tukey’s post hoc analysis. The experiments were performed three times, with similar results. The graphs depict one of three experiments.