Fig. 2.
AVR1-CO39 competes with RGA5HMA self-interaction for RGA5_S-binding in vitro. (A) Gel filtration traces showing the retention volume and SDS/PAGE gels of relevant elution fractions of dSP-AVR1-CO39, RGA5_S, and a 1:1 mixture of both proteins. The molecular weight of the recombinant proteins is depicted on the Top of the panels. (B) Sedimentation coefficient distributions calculated from sedimentation velocity analytical ultracentrifugation with dSP-AVR1-CO39, RGA5_S, and a 1:1 mixture of both proteins. These GF (A) and SV-AUC (B) experiments were performed three times with similar results.