Fig. 5.

BMP6 promotes astroglial differentiation of cultured adult hippocampal NSPCs. (A) Canonical signaling activation in cultured NSPCs after BMP6 treatment. (B) In vitro cell growth kinetics at 0, 4, 7, and 10 d (DIV). BMP6 impaired the expansion of NSPC cultures (*P < 0.05, ***P < 0.001). (C) Postmitotic astroglial differentiation (GFAP+BrdU−) of primary NSPC cultures after BMP6 treatment. (D) Percentage of GFAP+ and BrdU+ cells after BMP6 treatment. BMP6 impairs proliferation (0% BrdU+, **P < 0.01) and induces astroglial differentiation (% GFAP+, **P < 0.01). Data correspond to the average ± SEM, n = 3. (Scale bars, 10 μm in A and 20 μm in B.)