Fig. 1.

Transcriptional profiling of microdissected CPPs identifies a critical role for Tbx5 in pulmonary specification and lung development. (A, Left) Demonstration of microdissection methodology used for embryonic mouse experiments on an E9.5 embryo probed for Tbx5 RNA by ISH. (Scale bars: 0.25 mm.) (Right) Transcriptional profiling strategy used to measure the Tbx5-dependent transcriptome in the CPP-containing tissue by RNA-seq. (B) Spearman’s correlation of RNA-seq replicates. (C) Volcano plot of transcriptional profiling results with significantly dysregulated genes (teal) from the comparison of Tbx5^+/+ and Tbx5^−/− CPPs. Early markers of the PE and canonical Wnt and Shh signaling are identified. (D) qRT-PCR validation of 16 early lung-development genes that were significantly dysregulated in the RNA-seq.