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. 2018 Oct 19;115(45):11513–11518. doi: 10.1073/pnas.1716689115

Fig. 5.

Fig. 5.

Replication fitness of DENV2 tertiary structure mutants and control. (A) DENV2-positive BHK-21 cells after transfection of WT or mutant in vitro transcribed DENV2 RNAs. Virus was visualized by immunostaining of DENV2 envelope protein and nuclei (n ≥3, with ≥1,000 cells counted per condition). (B) Representative fields showing DENV2 RNA-transfected cells (green) and nuclei (DAPI, blue) at 72 h posttransfection. (C) Viral titer relative to WT following transfection of DENV2 RNA at 72 h (n ≥3). (D) Percent DENV2 RNA relative to WT at 72 h posttransfection as quantified by RT-qPCR (n ≥3). Values plotted are mean ± SEM of at least three independent experiments. Pol−, a lethal mutation in the DENV2 NS5 RNA-dependent RNA polymerase; n.s., not significant. *P < 0.05; **P < 0.01; ***P < 0.001, two-way ANOVA with Bonferroni correction in A, Student’s t test in C and D.