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. 2018 Nov 13;8:16750. doi: 10.1038/s41598-018-34755-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Administration of miR-182 affects Nav1.7 mRNA and protein levels in primary cultured DRG cells. (A) SCN9A mRNA and miR-182 expression in normal and TNF-α stimulated DRG cells after transfected with miR-182 agomir, miR-182 antagomir or scramble. (B) Level of Nav1.7 protein in normal and TNF-α stimulated DRG cells after transfected with miR-182 agomir or scramble. One-way ANOVA followed by post hoc Tukey, F_4,10 = 65.06 (C) Level of Nav1.7 protein increased after transfected with miR-182 antagomir. One-way ANOVA followed by post hoc Tukey, F_2,6 = 71.86, *P < 0.05, **P < 0.01 vs. the naive group; ^##P < 0.01 vs. the TNF-α group. n = 3 for each treatment.