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. 2018 Nov 13;8:16715. doi: 10.1038/s41598-018-35137-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview and concept of LT-FCM. (a) Schematic drawing of the optical setup of the LT-FCM. Optical components are indicated: (D)M refers to (dichroic) mirrors, BP/LP to band pass/long pass filters, ND to the neutral density filter, and BS to the beam splitter. The detection channels are LT (lifetime), SSC (side scatter), FL1/2 (fluorescence). Details on the optical components can be found in the Supplementary Information, Table S1. (b) Block diagram of the novel signal processing unit for single photon lifetime analysis for LT-FCM. (c) Visualisation of lifetime encoding with beads: Each code should exhibit similar spectral properties but differ in the decay kinetics from the other codes.