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. 2018 Nov 13;8:16776. doi: 10.1038/s41598-018-34960-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(c)mRNA^hCFTR and pDNA^hCFTR mediated expression of hCFTR in vitro (A) Total expression of hCFTR (calculated by multiplying positive cells (dots) and MFI (bars)) 24 h after transfection with 1 µg (c)mRNA^hCFTR and equivalent nmols of pDNA^hCFTR detected by flow cytometry. (B) Total expression of hCFTR 72 h after transfection with 1 µg (c)mRNA^hCFTR and equivalent nmols of pDNA^hCFTR detected by flow cytometry. (C) Western Blots, semi-quantifying human CFTR in transfected CFBE41o- cells, normalized to GAPDH and put relative to CFTR levels in 16HBE14o- cells. Blot section cropped from different blots are delineated with clear dividing lines (black) and full blot of same exposure time (30 mins) are depicted in Supplement Fig. S4. All bar graph data are depicted as means ± SDs while box plots data are depicted as the means ± minimum to maximum values. *P ≤ 0.05 versus unmodified mRNA^hCFTR; ^§P ≤ 0.05 and ^§§P ≤ 0.01 vs. pDNA^hCFTR.