Figure 6.

Expression of hCFTR protein in mouse lungs and delivery of cmRNA^hCFTR and pDNA^hCFTR in lungs. (A,D) All mouse groups, particles and particle combinations depicted in the study plan are color-coded for their treatment schemes, including dosage and application routes. (B,E) hCFTR ELISA, detecting specifically human CFTR, was performed on lung preparations at day 6 from Cftr^−/− mice treated twice via i.v. (B) or i.t. (E) route and measured 72 hours after the 2^nd instillment (endpoint); the same n = 4–7 mice per group were used. (C,F) Relative amounts of differently modified hCFTR mRNAs in the lungs applied i.v. or i.t., then determined by RT-quantitative PCR, compared to untreated Cftr^−/− mice (*P ≤ 0.05); n = 4–7 mice per group. All bar graph data are depicted as means ± SDs while box plots data are depicted as the means ± minimum to maximum values. The blue area represents the variance of the negative controls which are biological replicates. *P ≤ 0.05; **P ≤ 0.01 and ***P ≤ 0.001 versus untreated Cftr^−/− mice.