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. 2018 Nov 13;8:16776. doi: 10.1038/s41598-018-34960-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(c)mRNA^hCFTR and pDNA^hCFTR mediated immunogenicity in vivo Mice were i.v. or i.t. injected with a mix of (c)mRNA and NPs at a 1:10 ratio and ELISAs were performed post-i.v./i.t.-injection at three different time points. n.d., not detectable. The red dotted lines in (A,B) mark the detection limit as specified in the respective ELISA kit. All bar graph data are depicted as the means ± SD and box plots data are represented as the means ± minimum to maximum values.