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. 2018 Nov 13;8:16766. doi: 10.1038/s41598-018-35176-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq analysis of embryonic chicken feathers, chicken scutate scales and alligator overlapping scales. (A) Comparing the chicken feather-associated gene expression profile among chicken feathers (E7 and E9), chicken scutate scales (E9 and E11) and alligator overlapping scales (Es19 and Es22). The X-axis is the average CPM (count per million) value of feather-associated genes (Y-axis) from high to low in feather samples (left panel). Chicken scale (middle panel) and alligator scale (right panel) expression levels of feather-associated gene are shown. (B) Comparing the chicken scale-associated gene expression profile among chicken feathers (E7 and E9), chicken scutate scales (E9 and E11) and alligator overlapping scales (Es19 and Es22). The X-axis is the average CPM (count per million) value of scale-associated genes (Y-axis) from high to low in chicken scutate scale samples (middle panel). Chicken feather (left panel) and alligator scale (right panel) expression levels of scale-associated gene are shown. (C) Violin plots showing the chicken feather-associated genes are down-regulated in chicken scales and alligator scales. (D) Violin plot analysis showing the chicken scale-associated genes are down-regulated in both chicken feathers and alligator scales.