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. 2018 Nov 13;8:16766. doi: 10.1038/s41598-018-35176-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of the stem cell marker, K15 and differentiation marker, K75 in chicken and alligator scales. (A,A’) Expression of K15 in resting phase feather follicles. (B,B’) Expression of K75 in resting phase feather follicles. (C–F) Chicken scutate scales. (C,D) Expression of K15 in chicken scutate scales. (C) Hinge; (D) outer surface. (E,F) Expression of K75 in chicken scutate scales. (E) Hinge; (F) outer surface. (G–J) Alligator overlapping scales. (G,H) Expression of K15 in alligator overlapping scales. (G) Hinge; (H) outer surface. (I,J) Expression of K75 in alligator overlapping scales. (I) Hinge; (J) outer surface. Note K15 is positive in the hinge basal layer (C,G) whereas K75 is positive in the outer surface suprabasal layer (F,J). (K) Schematic drawing of the distribution of K15 (green), K75 (blue) and LRCs (red dot) in feathers (resting stage) and scales. dp, dermal papilla.