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. 2018 Nov 13;8:16716. doi: 10.1038/s41598-018-34699-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Longitudinal sections of the PLLA nerve guide four weeks after sciatic injury and repair. Panel (A–E): Neurofilament (NF), ED1, and S-100 staining of (A) regeneration matrix in a hollow silicone tube; (B) nerve guide without cells; (C–E) and nerve guide with cells. Panel F–H: LipidTOX Red (LTR) staining of PLLA nerve guide + cells. Immunofluorescence microscopy rendered a faint background for both polymer types, which is due to light scattering in the fiber matrix and not due to autofluorescence.