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. 2018 Nov 13;8:16742. doi: 10.1038/s41598-018-34918-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SaPI cycle after prophage induction. (1) SOS response after mitomycin C addition. (2) The prophage is induced and start the gene transcription, while the SaPI resides passively in the genome under the control of the global repressor Stl. (3) The interaction between the Stl and a specific phage derepressor protein induces the SaPI cycle. (4) The SaPI gene transcription starts. (5) SaPI is excised from the chromosome in covalently closed circular (CCC) state and (6) starts the replication. (7) The phage synthetises the procapsids and tails. (8) SaPI L indicates linear monomers released from the phage heads if the prophage is able to mobilise the SaPI.