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. 2018 Nov 13;8:16742. doi: 10.1038/s41598-018-34918-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SaPI induction of S. aureus clinical strains by endogenous phages. In total, 14 strains were treated with mitomycin C (2 µg/mL) to activate the SOS response and induce the resident prophages. Samples were isolated at 0 and 90 minutes, separated in agarose gel and blotted with the SaPI integrase-specific probe. The upper band corresponds to chromosomal bulk DNA including SaPI, and the lower bands correspond to covalently closed circular SaPI (CCC) excised from the genome or SaPI monomers released from the phage capsid (L) if the helper phage mediated induction and packaging.