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. 2018 Nov 13;8:16742. doi: 10.1038/s41598-018-34918-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Diagram of the ɸSa2mw genome with the cloned regions marked. (B) β-lactamase activity assay of the derivative pGTM3 (pCU1_Pcad) with different regions of φSa2mw in GTM754 (RN4220 φSa2mw pGTM2) strains. (C) Southern blot of GTM805 containing SaPImw2 and complemented with a plasmid overexpressing DUF313_ɸSa2mw. One millilitre of the culture was collected after 2 h of induction with 2 μM of CdCl₂. The lower band corresponds to CCC because there was no helper phage present. Southern blot was performed with the wild-type strains (GTM750 and GTM753) and the DUF3113 mutant deletion in MW2 (GTM756) and GTM753 (GTM757). Both Southern blots were hybridised with a SaPI integrase type III probe.