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. 2018 Nov 13;8:16742. doi: 10.1038/s41598-018-34918-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Sequence alignment of DUF3113 homologues in S. aureus phages using PRALINE⁴⁵. The scoring scheme assigns 0 as the least-conserved alignment position, with values up to 10 (asterisk) for the most-conserved alignment position (B). Southern blot of SaPImw2 excision after induction of 3xFLAG-tagged homologues to the DUF3113 protein from different phages cloned into pCN51 after 2 h of CdCl₂ treatment. (C) Western blot of 3xFLAG-tagged DUF3113 homologues cloned into pGTM3 (pCU1_ Pcad) with the same RBS (D). Sequence alignment of DUF3113 homologues of different staphylococcal species.