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. 2018 Nov 12;19:814. doi: 10.1186/s12864-018-5208-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Apoptosis, proliferation and cycle analysis of buffalo granulosa cells (GCs) after transfection with IGFBP7 ssRNAi. a Expression of IGFBP7in buffalo GCs detected by reverse transcription polymerase chain reaction. Line 1 and 2 correspond to IGFBP7 and GADPH. b The mRNA expression level of IGFBP7 after transfection with IGFBP7 ssRNAi. c Analysis of buffalo GCs proliferation after knockdown of IGFBP7 with the absorbance at 450 nm. d and e showed analysis of cycles and apoptosis of buffalo granulosa cells after transfection with IGFBP7 ssRNAi, respectively. The values in each bar represents the mean ± SEM (n = 3). The different superscripts and the symbol of * on bars showed significant level (P < 0.05)