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. 2018 Nov 12;18:178. doi: 10.1186/s12935-018-0675-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — TEAD4 expression pattern during HNSCC tumorigenesis in 4NQO-induced animal model. A Experimental scheme of 4NQO-induced HNSCC animal model. B–I Immunohistochemical staining of TEAD4 in samples from diverse stages in 4NQO-induced animal model. Images in the upper panel (B, D, F, H) were representative staining of TEAD4 in normal, epithelial with hyperplasia, epithelial with severe dysplasia/carcinoma in situ and squamous cell carcinoma, respectively. Images in the lower panel (C, E, G, I) were magnified from the black box area in the B, D, F, H images in the upper panel, respectively. Scale bar: 100 μm. J The mRNA levels of TEAD4 during the 4NQO-induced HNSCC were measured by qRT-PCR in pre-stored samples (n = 5, 6 samples per group). *P < 0.05, **P < 0.01, ANOVA analyses