Table 3.
Whey-derived peptides identified by LC-ESI-TOF–MS/MS in the LMW peptides (< 7 kDa) fraction of the 48 h whey fermentates of whey by P. acidilactici SDL1414
| Protein name | Peptide sequence | Prec MW | Prec m/z | Theor MW | Theor m/z | z |
|---|---|---|---|---|---|---|
| αS1-CN | (f10–23) GLPQEVLNENLLRF | 1640.9009 | 821.4577 | 1640.8885 | 821.4516 | 2 |
| (f10–22) GLPQEVLNENLLR | 1493.8313 | 747.9229 | 1493.8202 | 747.9174 | 2 | |
| (f1–23) RPKHPIKHQGLPQEVLNENLLRF | 3281.7998 | 821.4572 | 3286.8779 | 822.7267 | 4 | |
| (f14-23) EVLNENLLRF | 1245.6793 | 623.8469 | 1245.6718 | 623.8431 | 2 | |
| (f10-21) GLPQEVLNENLL | 1337.7279 | 669.8712 | 1337.7191 | 669.8668 | 2 | |
| (f24-34) FVAPFPEVFGK | 1236.6609 | 619.3377 | 1236.6543 | 619.3344 | 2 | |
| (f25-34) VAPFPEVFGK | 1089.5898 | 545.8022 | 1089.5859 | 545.8002 | 2 | |
| (f24-38) FVAPFPEVFGKEKVNEL | 1949.0464 | 650.6894 | 1949.0299 | 650.6839 | 3 | |
| (f80-98) HIQKEDVPSERYLGYLEQL | 2316.1978 | 580.0567 | 2316.175 | 580.051 | 4 | |
| β-CN | (f1-27) RELEELNVPGEIVESL | 1823.9845 | 912.9995 | 1824.9469 | 913.4807 | 2 |
| (f1-25) RELEELNVPGEIVE | 1623.8574 | 812.936 | 1624.8308 | 813.4227 | 2 | |
| (f1-22) RELEELNVPGE | 1283.6456 | 642.8301 | 1283.6357 | 642.8251 | 2 | |
| (f1-24) RELEELNVPGEIV | 1494.8132 | 748.4139 | 1494.8042 | 748.4094 | 2 | |
| (f192-209) LYQEPVLGPVRGPFPIIV | 1993.1543 | 665.392 | 1993.14 | 665.3873 | 3 | |
| (f193-209) YQEPVLGPVRGPFPIIV | 1880.0715 | 941.043 | 1880.056 | 941.0353 | 2 | |
| (f193-208) YQEPVLGPVRGPFPII | 1781.0021 | 891.5083 | 1780.9875 | 891.5011 | 2 | |
| (f194-209) QEPVLGPVRGPFPIIV | 1699.9836 | 850.9991 | 1699.9662 | 850.9904 | 2 | |
| (f195-209) EPVLGPVRGPFPIIV | 1588.9463 | 795.4804 | 1588.9341 | 795.4743 | 2 | |
| (f83-95) VVPPFLQPEVMGV | 1410.7693 | 706.3919 | 1410.7581 | 706.3864 | 2 | |
| κ-CN | (f161-169) TVQVTSTAV | 904.49048 | 453.2525 | 904.48657 | 453.2505 | 2 |
| (f155-169) SPPEINTVQVTSTAV | 1541.8065 | 771.9105 | 1541.7937 | 771.9041 | 2 | |
| (f149-169) SPEVIESPPEINTVQVTSTAV | 2276.0999 | 759.7072 | 2276.0825 | 759.7014 | 3 | |
| (f151-169) EVIESPPEINTVQVTSTAV | 2012.0497 | 671.6905 | 2012.0314 | 671.6844 | 3 | |
| (f159-169) INTVQVTSTAV | 1131.6196 | 566.8171 | 1131.6135 | 566.814 | 2 | |
| (f152-169) VIESPPEINTVQVTSTAV | 1883.0045 | 942.5095 | 1882.9888 | 942.5016 | 2 | |
| (f150-169) PEVIESPPEINTVQVTSTAV | 2109.0984 | 704.0401 | 2109.0842 | 704.0353 | 3 | |
| (f157-169) PEINTVQVTSTAV | 1357.7208 | 679.8677 | 1357.7089 | 679.8618 | 2 | |
| (f151-169) EVIESPPEINTVQVTSTAV | 2034.0271 | 679.0163 | 2034.0133 | 679.0117 | 3 | |
| (f151-165) EVIESPPEINTVQVT | 1653.8649 | 827.9397 | 1653.8462 | 827.9304 | 2 | |
| (f151-163) EVIESPPEINTVQ | 1453.7411 | 727.8778 | 1453.73 | 727.8723 | 2 | |
| (f149-162) SPEVIESPPEIN | 1309.6481 | 655.8313 | 1309.6401 | 655.8273 | 2 | |
| (f149-163) SPEVIESPPEINTVQ | 1717.8009 | 859.9077 | 1717.781 | 859.89 | 2 | |
| (f151-162) EVIESPPEIN | 1125.5629 | 563.7887 | 1125.5554 | 563.785 | 2 | |
| (f116-141) MAIPPKKNQDKTEIPTINTIASGEPT | 2793.4849 | 699.3785 | 2793.4583 | 699.3718 | 4 | |
| (f109-151)PPKKNQDKTEIPTINTIASGEPTSTPTTEAVESTVATLEDSPE | 4523.3027 | 1131.833 | 4523.299 | 1131.81 | 4 | |
| (f106-149)MAIPPKKNQDKTEIPTINTIASGEPTSTPTTEAVESTVATLED | 4525.3066 | 1132.334 | 4525.263 | 1132.32 | 4 | |
| β-lg | (f130-149) DEALEKFDKALKALPMHIRL | 2353.2981 | 471.6669 | 2353.2827 | 471.6638 | 5 |
| (f130-146) DEALEKFDKALKALPMH | 1955.031 | 489.765 | 1955.0187 | 489.7619 | 4 | |
| (f130-145) DEALEKFDKALKALPM | 1817.973 | 606.9983 | 1817.9597 | 606.9938 | 3 | |
| (f1-11) LIVTQTMKGLD | 1217.6791 | 609.8468 | 1217.6689 | 609.8417 | 2 | |
| (153-162) PTQLEEQCHI | 1162.5697 | 582.2921 | 1162.5619 | 582.2882 | 2 | |
| (147-156) IRLSFNPTQL | 1187.6759 | 594.8452 | 1187.6663 | 594.8404 | 2 | |
| (f1-11) LIVTQTMKGL | 1102.6472 | 552.3309 | 1102.642 | 552.3283 | 2 | |
| (f1-12) LIVTQTMKGLD | 1217.6791 | 609.8468 | 1217.669 | 609.842 | 2 | |
| (f153-162) PTQLEEQCHI | 1162.5697 | 582.2921 | 1162.562 | 582.288 | 2 | |
| (f147-156) IRLSFNPTQL | 1187.6759 | 594.8452 | 1187.666 | 594.84 | 2 | |
| (f1-10) LIVTQTMKGL | 1102.6472 | 552.3309 | 1102.624 | 552.328 | 2 | |
| Lactophorin (PP3) | (f1-18) ILNKPEDETHLEAQPTDA | 2019.999 | 674.3403 | 2019.9749 | 674.3323 | 3 |
| (f1-17) ILNKPEDETHLEAQPTD | 1948.9561 | 650.6593 | 1948.9377 | 650.6532 | 3 | |
| (f57-67) QPQSQNPKLPL | 1231.6656 | 616.8401 | 1231.6561 | 616.8353 | 2 | |
| (f54-67) SSRQPQSQNPKLPL | 1578.8573 | 527.293 | 1578.8478 | 527.2899 | 3 | |
| PIGR | (f383-404) PGRPTGYSGSSKALVSTLVPLA | 2157.1958 | 720.0726 | 2157.1794 | 720.0671 | 3 |
| UP (GP2) | (f455-473) SEGVAIDPARVLDLGPITR | 1978.1027 | 660.3748 | 1978.0847 | 660.3688 | 3 |
CN casein, m/z mass to charge ratio, where z number of positively charged ions, Prec precision, Theor theoretical
αS1-CN alpha-S1-casein, κ-CN kappa-casein, β-CN beta-casein, β-lg beta lactoglobulin, PP3 proteose-peptone component 3, UP (GP2) uncharacterized protein GP2; PIGR polymeric immunoglobulin receptor