Table 2.
The experimental conditions observed (actual and coded) for octyl octanoate synthesis using lipase bearing dead mycelia as biocatalyst
| Design point type | Experiment # | A: temperature (°C) | B: moisture (%) | C: sahking speed RPM | D: enzyme dose (I.U.) |
|---|---|---|---|---|---|
| Center | 3 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) |
| 4 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) | |
| 22 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) | |
| 23 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) | |
| 27 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) | |
| 29 | 37.5 (0) | 2 (0) | 150 (0) | 275 (0) | |
| Axial | 5 | 37.5 (0) | 0.2 (− β) | 150 (0) | 275 (0) |
| 11 | 37.5 (0) | 5.0 (+ β) | 150 (0) | 275 (0) | |
| 14 | 37.5 (0) | 2 (0) | 50 (− β) | 275 (0) | |
| 15 | 37.5 (0) | 2 (0) | 150 (0) | 50 (− β) | |
| 18 | 37.5 (0) | 2 (0) | 150 (0) | 500 (+ β) | |
| 20 | 50 (+ β) | 2 (0) | 150 (0) | 275 (0) | |
| 26 | 25 (− β) | 2 (0) | 150 (0) | 275 (0) | |
| 30 | 37.5 (0) | 2 (0) | 250 (+ β) | 275 (0) | |
| Factorial | 1 | 43.75 (+ 1) | 3 (+ 1) | 105 (− 1) | 387.5 (+ 1) |
| 2 | 31.25 (− 1) | 3 (+ 1) | 195 (+ 1) | 387.5 (+ 1) | |
| 6 | 31.25 (− 1) | 3 (+ 1) | 195 (+ 1) | 162.5 (− 1) | |
| 7 | 31.25 (− 1) | 1 (− 1) | 195 (+ 1) | 387.5 (+ 1) | |
| 8 | 43.75 (+ 1) | 1 (− 1) | 195 (+ 1) | 387.5 (+ 1) | |
| 9 | 31.25 (− 1) | 1 (− 1) | 195 (+ 1) | 162.5 (− 1) | |
| 10 | 43.75 (+ 1) | 1 (− 1) | 105 (− 1) | 387.5 (+ 1) | |
| 12 | 31.25 (− 1) | 1 (− 1) | 105 (− 1) | 162.5 (− 1) | |
| 13 | 43.75 (+ 1) | 1 (− 1) | 105 (− 1) | 162.5 (− 1) | |
| 16 | 31.25 (− 1) | 3 (+ 1) | 105 (− 1) | 162.5 (− 1) | |
| 17 | 31.25 (− 1) | 3 (+ 1) | 105 (− 1) | 387.5 (+ 1) | |
| 19 | 43.75 (+ 1) | 3 (+ 1) | 195 (+ 1) | 387.5 (+ 1) | |
| 21 | 43.75 (+ 1) | 1 (− 1) | 195 (+ 1) | 162.5 (− 1) | |
| 24 | 43.75 (+ 1) | 3 (+ 1) | 195 (+ 1) | 162.5 (− 1) | |
| 25 | 43.75 (+ 1) | 3 (+ 1) | 105 (− 1) | 162.5 (− 1) | |
| 28 | 31.25 (− 1) | 1 (− 1) | 105 (− 1) | 387.5 (+ 1) |