Table 3.
The summary of the validation experiments conducted observing the most suitable reaction conditions for octyl octanoate synthesis using lipase bearing dead mycelia as biocatalyst
| Biocatalyst | Carrier | Immobilization | Reaction media | Acylating agent | Substrate | Reaction conditions | Acylation activity | Ocyloctanoate after 24 h | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Rhizopus arrhizus Lipases | Dead Mycelia | Cell immobilization | n-hexane | Octanoic acid | Octanol | Temperature | Moisture | Shaking speed | Enzyme dose | μmol L−1 min−1 | (%) |
| – | – | – | – | 750 mM | 850 mM | 35 °C | 3% | 150 RPM | 250 I.U. | 148.25 | 70.08 |
| – | – | – | – | – | – | – | – | – | – | 149.14 | 71.24 |
| – | – | – | – | – | – | – | – | – | – | 150.61 | 71.14 |
| Mean ± SD | 149.34 ± 1.15 | 70.82 ± 0.64 | |||||||||