Table 4.
The percentage of major lymphocyte subpopulations in the peripheral blood of mature female and male Wistar rats on the first day after the administration of low- or high-dose LPS
| Lymphocyte subpopulation, % | Females | Males | P-value | F-value | ||||
|---|---|---|---|---|---|---|---|---|
| Control (n=10) | LPS 1.5 mg/kg (n=9) | LPS 15 mg/kg (n=10) | Control (n=10) | LPS 1.5 mg/kg (n=6) | LPS 15 mg/kg (n=10) | |||
| T cells (CD3+) | 47.81 (42.3–56.4) | 42.52 (29.7–47.5) | 39.43 (30.7–52.7) | 53.01* (45.5–63.0) | 43.72 (38.1–53.2) | 23.23 (18.0–29.0) | 0.00083–3 | 503 |
| T-helper cells (CD3+ CD4+) | 47.14 (29.0–54.9) | 56.25 (47.9–59.1) | 65.26 (37.6–67.4) | 40.74 (33.7–45.0) | 44.75 (34.6–49.5) | 70.26 (60.5–73.0) | 0.055–5 0.026–6 | 692 |
| Cytotoxic T cells (CD3+ CD8a+) | 52.07 (44.0–70.8) | 40.88 (38.5–51.3) | 32.559 (26.8–46.3) | 59.97 (51.8–71.4) | 47.78 (46.5–63.6) | 28.69 (26.4–29.5) | 0.0039–9 | 396 |
| B cells (CD45+) | 15.310 (12.5–19.3) | 16.811 (12.5–23.3) | 18.212 (15.5–22.4) | 19.510 (15.5–21.7) | 25.811 (19.1–29.1) | 16.212 (13.0–24.8) | 346 | |
| NK cells (CD314+) | 4.913 (3.0–6.1) | 22.414 (18.9–27.5) | 26.315 (16.2–52.2) | 5.613 (2.9–9.6) | 30.814 (20.9–40.8) | 28.315 (21.0–38.7) | 165 | |
| Activated T-helper cells (CD4+ CD25+ Foxp3–) | 7.816 (5.0–11.2) | 6.417 (6.0–9.4) | 4.118 (2.8–5.0) | 3.316 (2.4–4.7) | 3.517 (2.0–4.6) | 2.618 (2.1–3.1) | 0.00216–16 0.00117–17 | 79 |
| Regulatory T cells (CD4+ CD25+ Foxp3+) | 3.818 (3.4–4.6) | 4.220 (2.3–4.3) | 2.221 (1.5–2.7) | 3.719 (3.6–4.8) | 4.220 (3.3–5.2) | 2.421 (1.4–3.2) | 370 | |
| Statistical significance of differences (P) | 0.034–5 0.027–8 |
0.000213–14 0.000213–15 0.00218–21 |
0.0420–21 |
0.051–3 0.0022–3 0.025–6 0.027–8 |
0.00057–9 0.018–9 0.000213–14 0.000113–15 |
0.0418–21 0.0317–18 0.0420–21 |
||
Notes: Superscript 1-21 indicates the group number. Superscripts n=# is the number of observations in the group. Data shown as median (interquartile range).
Abbreviations: LPS, lipopolysaccharide; Me, median; NK, natural killer.