Table 5.
Serum TGF-β level and cytokine production by ConA-activated spleen cells in mature female and male Wistar rats on the first day after the administration of low- or high-dose LPS
| Sex | Group | TGF-β, ng/mL | IL-2, pg/mL | IL-4, pg/mL | IFNγ, pg/mL | TNF, pg/mL |
|---|---|---|---|---|---|---|
| Females | Control1 | 79.0 (63.2–81.9)n=5 | 4,980 (4,700–5,318)n=6 | 300 (284–374)n=7 | 378.9 (259.3–425,8)n=9 | 462.8 (354.8–568.0)n=10 |
| LPS 1.5 mg/kg2 | 23.0 (13.0–25.7)n=7 | 1,850 (1,295–2,220)n=5 | 250 (164–301)n=9 | 371.7 (190.2–501.4)n=5 | 850.2 (785.4–920.1)n=9 | |
| LPS 15 mg/kg3 | 37.2 (17.5–41.0)n=5 | 5,400 (5,100–5,400)n=5 | 86.8 (46.0–127.1)n=5 | 375.0 (320.3–421.9)n=6 | 172.7 (135.7–202.4)n=6 | |
| Males | Control4 | 50.8 (43.0–64.0)n=5 | 3,107 (2,216–5,953)n=7 | 252 (220–356)n=8 | 421.9 (359.4–1164)n=10 | 301.3 (237.8–385.6)n=8 |
| LPS 1.5 mg/kg5 | 20.5 (13.0–41.8)n=6 | 4,934 (4,361–6,950)n=6 | 46 (43–62.9)n=6 | 266.1 (228.1–382.4)n=6 | 209.0 (167.6–488.7)n=6 | |
| LPS 15 mg/kg6 | 20.2 (19.3–29.4)n=5 | 1,704 (1,257–2,682)n=5 | 17.8 (16.5–17.9)n=5 | 300.1 (206.5–351.6)n=6 | 100.8 (56.5–156.0)n=6 | |
| Statistical significance of differences (P) |
0.00051–2 0.0091–3 0.034–5 0.0094–6 |
0.0081–2 0.041–3 0.0052–3 0.044–5 0.044–6 0.015–6 0.012–5 0.0083–6 |
0.061–3 0.072–3 0.0054–5 0.044–6 0.00025–6 0.012–5 0.0073–6 |
0.074–5 0.044–6 0.045–6 0.021–4 |
0.021–2 0.021–3 0.012–3 0.024–6 0.025–6 0.021–4 0.022–5 |
|
| F-value | 198 | 299 | 151 | 24 | 63 | |
Notes: Superscript 1-6 indicates the group number. Superscripts n=# is the number of observations in the group. Data shown as median (interquartile range).
Abbreviations: IFNγ, interferon γ; LPS, lipopolysaccharide; TGF-β, transforming growth factor-β; Me, median.